• Product nameAnti-Frizzled 4 antibody
    See all Frizzled 4 primary antibodies
  • Description
    Goat polyclonal to Frizzled 4
  • SpecificityTested in human and expected to react in rat given sequence similarity
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 39-51 of Human Frizzled 4 (NP_036325.2).

  • Positive control
    • Approx 60kDA band observed in Human Colon and Small Intestine



Our Abpromise guarantee covers the use of ab77724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/16000.


  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificityAlmost ubiquitous. Largely expressed in adult heart, skeletal muscle, ovary, and fetal kidney. Moderate amounts in adult liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, adult lung, prostate, testis, colon, fetal brain and liver.
  • Involvement in diseaseDefects in FZD4 are the cause of vitreoretinopathy exudative type 1 (EVR1) [MIM:133780]; also known as autosomal dominant familial exudative vitreoretinopathy (FEVR) or Criswick-Schepens syndrome. EVR1 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationMembrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 344 antibody
    • CD 344 antigen antibody
    • CD344 antibody
    • CD344 antigen antibody
    • EVR1 antibody
    • FEVR antibody
    • Frizzled (Drosophila) homolog 4 antibody
    • Frizzled 4 antibody
    • Frizzled family receptor 4 antibody
    • Frizzled homolog 4 (Drosophila) antibody
    • Frizzled-4 antibody
    • Fz 4 antibody
    • Fz-4 antibody
    • Fz4 antibody
    • FZD4 antibody
    • FZD4_HUMAN antibody
    • FZD4S antibody
    • FzE4 antibody
    • GPCR antibody
    • hFz4 antibody
    • MGC34390 antibody
    • WNT receptor frizzled 4 antibody
    see all

Anti-Frizzled 4 antibody images

  • Anti-Frizzled 4 antibody (ab77724) at 1 µg/ml + Small Intestine (Ileum) cell lysate at 35 µg

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-Frizzled 4 antibody (ab77724)

This product has been referenced in:
  • Formosa A  et al. MicroRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32.31 locus, regulate proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells. Oncogene N/A:N/A (2013). Human . Read more (PubMed: 24166498) »

See 1 Publication for this product

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