• Product nameAnti-Frizzled 5 antibody
    See all Frizzled 5 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled 5
  • SpecificityRecognizes human Fz5.
  • Tested applicationsSuitable for: ICC/IF, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to N terminal amino acids 564-585 of Human Frizzled 5.



Our Abpromise guarantee covers the use of ab14475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20530549
IHC-FoFr Use at an assay dependent concentration. PubMed: 20530549
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.


  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainThe PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationCell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2orf31 antibody
    • DKFZP434E2135 antibody
    • Frizzled 5 seven transmembrane spanning receptor antibody
    • Frizzled class receptor 5 antibody
    • Frizzled family receptor 5 antibody
    • Frizzled homolog 5 antibody
    • Frizzled-5 antibody
    • Frizzled5 antibody
    • Fz 5 antibody
    • Fz-5 antibody
    • Fz5 antibody
    • FZD 5 antibody
    • fzd5 antibody
    • FZD5_HUMAN antibody
    • FzE 5 antibody
    • FzE5 antibody
    • HFZ 5 antibody
    • hFz5 antibody
    • MGC129692 antibody
    • Seven transmembrane receptor frizzled 5 antibody
    • Uncharacterized protein C2orf31 antibody
    • Wnt receptor antibody
    see all

Anti-Frizzled 5 antibody images

  • Predicted band size : 63 kDa

References for Anti-Frizzled 5 antibody (ab14475)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification Prostate cancer
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde

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Submitted Nov 13 2015

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Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab regarding this antibody.

The predicted molecular weight is based only on the precursor amino acid sequence of Frizzled 5,...

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Thank you for your enquiry. We currently have three antibodies against Frizzled 5. Further to clarification with the lab, only one of them has been tested for use in western blot; Frizzled 5 antibody (ab14475). I have uploaded an image of this immu...

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