Anti-Frizzled 5 antibody (ab75234)
Key features and details
- Rabbit polyclonal to Frizzled 5
- Suitable for: ELISA, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Frizzled 5 antibody
See all Frizzled 5 primary antibodies -
Description
Rabbit polyclonal to Frizzled 5 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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ICC/IF |
1/500 - 1/1000.
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IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ICC/IF
1/500 - 1/1000. |
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Domain
The PDZ-binding motif mediates interaction with GOPC.
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi. - Information by UniProt
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Database links
- Entrez Gene: 7855 Human
- Entrez Gene: 14367 Mouse
- Entrez Gene: 317674 Rat
- Omim: 601723 Human
- SwissProt: Q13467 Human
- SwissProt: Q9EQD0 Mouse
- SwissProt: Q8CHL0 Rat
- Unigene: 17631 Human
see all -
Alternative names
- C2orf31 antibody
- DKFZP434E2135 antibody
- Frizzled 5 seven transmembrane spanning receptor antibody
see all
Images
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All lanes : Anti-Frizzled 5 antibody (ab75234) at 1/500 dilution
Lane 1 : LOVO cell extracts
Lane 2 : LOVO cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. -
ab75234 at 1/500 dilution staining Frizzled 5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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ab75234 (4µg/ml) staining Frizzled 5 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the Islets of Langerhans.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab75234 has been referenced in 17 publications.
- Niu J et al. Oligodendroglial ring finger protein Rnf43 is an essential injury-specific regulator of oligodendrocyte maturation. Neuron 109:3104-3118.e6 (2021). PubMed: 34390652
- Ding J et al. CircNPM1 strengthens Adriamycin resistance in acute myeloid leukemia by mediating the miR-345-5p/FZD5 pathway. Cent Eur J Immunol 46:162-182 (2021). PubMed: 34764785
- Ning X et al. The Levels of Wnt5a and Its Receptors Frizzled5 and Frizzled2 as Immunohistochemical Biomarkers of Severity of Psoriasis. Clin Cosmet Investig Dermatol 14:1651-1656 (2021). PubMed: 34785924
- Prodromidou K et al. MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development. Elife 9:N/A (2020). PubMed: 32459171
- Sun Y et al. FZD5 contributes to TNBC proliferation, DNA damage repair and stemness. Cell Death Dis 11:1060 (2020). PubMed: 33311446