Overview

  • Product name
  • Description
    Rabbit polyclonal to Frizzled 5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Frizzled 5.

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates: LOVO; PC3.

Properties

Applications

Our Abpromise guarantee covers the use of ab96547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 65 kDa).

Target

  • Function
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2orf31 antibody
    • DKFZP434E2135 antibody
    • Frizzled 5 seven transmembrane spanning receptor antibody
    • Frizzled class receptor 5 antibody
    • Frizzled family receptor 5 antibody
    • Frizzled homolog 5 antibody
    • Frizzled-5 antibody
    • Frizzled5 antibody
    • Fz 5 antibody
    • Fz-5 antibody
    • Fz5 antibody
    • FZD 5 antibody
    • fzd5 antibody
    • FZD5_HUMAN antibody
    • FzE 5 antibody
    • FzE5 antibody
    • HFZ 5 antibody
    • hFz5 antibody
    • MGC129692 antibody
    • Seven transmembrane receptor frizzled 5 antibody
    • Uncharacterized protein C2orf31 antibody
    • Wnt receptor antibody
    see all

Images

  • All lanes : Anti-Frizzled 5 antibody (ab96547) at 1 µg/ml

    Lane 1 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : PC3 whole cell lysate (ab3954)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References

ab96547 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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