Overview

  • Product name
  • Description
    Rabbit polyclonal to Frizzled 6
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 500-550 of Human Frizzled 6 (NP_003497.2; GeneID 8323).

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 2 - 5 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
    • Tissue specificity
      Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.
    • Involvement in disease
      Nail disorder, non-syndromic congenital, 10
      Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.
    • Sequence similarities
      Belongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.
    • Domain
      Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
      The FZ domain is involved in binding with Wnt ligands.
    • Post-translational
      modifications
      Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
    • Cellular localization
      Membrane. Cell membrane. Cell surface. Apical cell membrane. Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity).
    • Information by UniProt
    • Database links
    • Alternative names
      • Frizzled 6 seven transmembrane spanning receptor antibody
      • Frizzled drosophila homolog of 6 antibody
      • Frizzled family receptor 6 antibody
      • Frizzled homolog 6 (Drosophila) antibody
      • Frizzled homolog 6 antibody
      • Frizzled-6 antibody
      • Frizzled6 antibody
      • Fz 6 antibody
      • Fz-6 antibody
      • Fz6 antibody
      • FZD 6 antibody
      • Fzd6 antibody
      • FZD6_HUMAN antibody
      • Hfz 6 antibody
      • hFz6 antibody
      • NDNC10 antibody
      see all

    Images

    • Frizzled 6 in HeLa whole cell lysate immunoprecipitated using ab105116 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 3 seconds. For blotting immunoprecipitated Frizzled 6, another antibody was used at 0.4 µg/ml.

    References

    ab105116 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab105116.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up