• Product nameAnti-Frizzled 6 antibody
    See all Frizzled 6 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled 6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Frizzled 6.

    (Peptide available as ab108466.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Kidney (Human) Tissue Lysate; Heart (Human) Tissue Lysate; Skeletal Muscle (Human) Tissue Lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab98180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa).

Use a concentration of 1μg/ml. Detects a band approximately 80kDa.


  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
  • Tissue specificityDetected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.
  • Involvement in diseaseNail disorder, non-syndromic congenital, 10
    Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localizationMembrane. Cell membrane. Cell surface. Apical cell membrane. Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled 6 seven transmembrane spanning receptor antibody
    • Frizzled drosophila homolog of 6 antibody
    • Frizzled family receptor 6 antibody
    • Frizzled homolog 6 (Drosophila) antibody
    • Frizzled homolog 6 antibody
    • Frizzled-6 antibody
    • Frizzled6 antibody
    • Fz 6 antibody
    • Fz-6 antibody
    • Fz6 antibody
    • FZD 6 antibody
    • Fzd6 antibody
    • FZD6_HUMAN antibody
    • Hfz 6 antibody
    • hFz6 antibody
    • NDNC10 antibody
    see all

Anti-Frizzled 6 antibody images

  • All lanes : Anti-Frizzled 6 antibody (ab98180) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 79 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-Frizzled 6 antibody (ab98180)

ab98180 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E9.5 mouse embryo section)
Permeabilization Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Specification E9.5 mouse embryo section
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde

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Submitted Jan 30 2015