Anti-Frizzled 6 antibody - N-terminal (ab150545)

Overview

  • Product name
    Anti-Frizzled 6 antibody - N-terminal
    See all Frizzled 6 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled 6 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human, Non human primates
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide, corresponding to 16 amino acid residues from the N terminal, extracellular domain of Human Frizzled 6 (NP_003497.2).

  • Positive control
    • Human pancreas tissue; HEK293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 6 - 14 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 3 µg/ml.

Target

  • Function
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.
  • Tissue specificity
    Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.
  • Involvement in disease
    Nail disorder, non-syndromic congenital, 10
    Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications
    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localization
    Membrane. Cell membrane. Cell surface. Apical cell membrane. Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled 6 seven transmembrane spanning receptor antibody
    • Frizzled drosophila homolog of 6 antibody
    • Frizzled family receptor 6 antibody
    • Frizzled homolog 6 (Drosophila) antibody
    • Frizzled homolog 6 antibody
    • Frizzled-6 antibody
    • Frizzled6 antibody
    • Fz 6 antibody
    • Fz-6 antibody
    • Fz6 antibody
    • FZD 6 antibody
    • Fzd6 antibody
    • FZD6_HUMAN antibody
    • Hfz 6 antibody
    • hFz6 antibody
    • NDNC10 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Frizzled 6 with ab150545 at 6 µg/ml.
  • Immunocytochemical analysis of HEK293 cells transfected with Frizzled 6 (left panel) and non-transfected cells (right panel).

References

ab150545 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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