Overview

  • Product name
  • Description
    Rabbit polyclonal to Frizzled 7
  • Tested applications
    Suitable for: ICC/IF, IHC-FoFr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Dog, Xenopus laevis, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human Frizzled 7.

    (Peptide available as ab64635.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Mouse Skeletal Muscle Tissue, Rat Spinal Cord Tissue, Human Brain Tissue, HEK293 Whole Cell, HeLa Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab64636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23251385
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 64 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificity
    High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled 7, seven transmembrane spanning receptor antibody
    • frizzled class receptor 7 antibody
    • Frizzled drosophila homolog of 7 antibody
    • Frizzled family receptor 7 antibody
    • Frizzled homolog 7 antibody
    • Frizzled-7 antibody
    • Frizzled7 antibody
    • Fz 7 antibody
    • Fz-7 antibody
    • Fz7 antibody
    • FZD 7 antibody
    • FZD7 antibody
    • FZD7_HUMAN antibody
    • FzE 3 antibody
    • FzE3 antibody
    • hFz 7 antibody
    • hFz7 antibody
    • Homolog of Drosophila Frizzled 7 antibody
    • OTTHUMP00000163742 antibody
    see all

Images

  • All lanes : Anti-Frizzled 7 antibody (ab64636) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Predicted band size : 64 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab64636 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64636, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.
  • ab64636 (1/250) staining Frizzled 7 in paraffin-embedded Human kidney (papillary cancer) tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  The secondary antibody was goat anti rabbit conjugated to HRP.  For further experimental details please refer to abreview.

    See Abreview

  • Immunohistochemical image of ab64636 (dilution 1/2000) staining in rat spinal cord PFA fixed frozen tissue section, performed on an Olympus BX61 Motorized Research Microscope. The scale bar represents 50 µm.

  • All lanes : Anti-Frizzled 7 antibody (ab64636) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutesFrizzled 7 has a predicted molecular weight of 64 kDa, however as it contains a number of potential glycosylation sites (SwissProt data) it is thought that this is the reason it is running at 90kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • IHC image of Frizzled 7 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64636, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References

This product has been referenced in:
  • Gammons MV  et al. Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay. J Cell Sci 129:3892-3902 (2016). Human . Read more (PubMed: 27744318) »
  • Yuan K  et al. Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis. Am J Pathol 185:69-84 (2015). Read more (PubMed: 25447046) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Breast carcinoma)
Specification
Breast carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 19 2014


The polyclonal anti-Frizzled 7 (ab64636) is an IgG. The isotype and allotype have not been determined.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (SCLC - NCI-H146)
Loading amount
80 µg
Specification
SCLC - NCI-H146
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney (papillary cancer))
Specification
Kidney (papillary cancer)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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