Overview

  • Product name
  • Description
    Rabbit polyclonal to Frizzled 8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 634-694 of Human Frizzled 8 (UniProt: Q9H461).

  • Positive control
    • H1299 cell lysate; Human SW480 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Function
    Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
  • Tissue specificity
    Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications
    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localization
    Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled 8 seven transmembrane spanning receptor antibody
    • frizzled 8, seven transmembrane spanning receptor antibody
    • Frizzled family receptor 8 antibody
    • frizzled homolog 8 (Drosophila) antibody
    • Frizzled homolog 8 antibody
    • Frizzled-8 antibody
    • FZ 8 antibody
    • Fz-8 antibody
    • FZ8 antibody
    • FZD 8 antibody
    • FZD8 antibody
    • FZD8_HUMAN antibody
    • hFZ 8 antibody
    • hFz8 antibody
    • HGNC4046 antibody
    • Homolog of Drosophila Frizzled 8 antibody
    see all

Images

  • Anti-Frizzled 8 antibody (ab155650) at 1/10000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SW480 xenograft tissue labeling Frizzled 8 using ab155650 at a 1/100 dilution.

References

This product has been referenced in:
  • Jin Y  et al. The Expression of miR-375 Is Associated with Carcinogenesis in Three Subtypes of Lung Cancer. PLoS One 10:e0144187 (2015). WB . Read more (PubMed: 26642205) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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