Overview

  • Product name
  • Description
    Rabbit polyclonal to Frizzled 8
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human Frizzled 8.

  • Positive control
    • Jurkat cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab75235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA 1/5000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23251385
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
  • Tissue specificity
    Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain
    The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications
    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localization
    Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled 8 seven transmembrane spanning receptor antibody
    • frizzled 8, seven transmembrane spanning receptor antibody
    • Frizzled family receptor 8 antibody
    • frizzled homolog 8 (Drosophila) antibody
    • Frizzled homolog 8 antibody
    • Frizzled-8 antibody
    • FZ 8 antibody
    • Fz-8 antibody
    • FZ8 antibody
    • FZD 8 antibody
    • FZD8 antibody
    • FZD8_HUMAN antibody
    • hFZ 8 antibody
    • hFz8 antibody
    • HGNC4046 antibody
    • Homolog of Drosophila Frizzled 8 antibody
    see all

Images

  • All lanes : Anti-Frizzled 8 antibody (ab75235) at 1/500 dilution

    Lane 1 : Jurkat cell extracts
    Lane 2 : Jurkat cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 30 kDa,48 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab75235 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75235, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab75235 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75235, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemical image of ab75235 (dilution 1/250) staining in rat spinal cord PFA fixed frozen tissue section, performed on an Olympus BX61 Motorized Research Microscope. The scale bar represents 50 µm.

References

This product has been referenced in:
  • Corbett L  et al. Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells. PLoS One 10:e0142794 (2015). WB ; Rat . Read more (PubMed: 26566235) »
  • Zhang N  et al. MicroRNA-100 promotes migration and invasion through mammalian target of rapamycin in esophageal squamous cell carcinoma. Oncol Rep 32:1409-18 (2014). Read more (PubMed: 25109390) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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