Use at an assay dependent concentration. PubMed: 23251385
Use a concentration of 5 µg/ml.
FunctionReceptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
Tissue specificityMost abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.
DomainThe PDZ-binding motif mediates interaction with GOPC. Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway. The FZ domain is involved in binding with Wnt ligands.
Post-translational modificationsUbiquitinated by ZNRF3, leading to its degradation by the proteasome.
Cellular localizationMembrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
ICC/IF image of ab75235 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75235, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab75235 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75235, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Frizzled 8 antibody (ab75235)Image from Gonzalez P et al., PLoS One. 2012;7(12):e50793. Fig 6.; doi: 10.1371/journal.pone.0050793. Epub 2012 Dec 10.
Immunohistochemical image of ab75235 (dilution 1/250) staining in rat spinal cord PFA fixed frozen tissue section, performed on an Olympus BX61 Motorized Research Microscope. The scale bar represents 50 µm.
References for Anti-Frizzled 8 antibody (ab75235)
This product has been referenced in:
Corbett L et al. Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells. PLoS One10:e0142794 (2015).
Read more (PubMed: 26566235) »
Gonzalez P et al. Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats. PLoS One7:e50793 (2012).
Read more (PubMed: 23251385) »