Recombinant
RabMAb

Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

Overview

  • Product name
    Anti-Frizzled 8 antibody [EPR7308(2)]
    See all Frizzled 8 primary antibodies
  • Description
    Rabbit monoclonal [EPR7308(2)] to Frizzled 8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Frizzled 8 aa 650-750. The exact sequence is proprietary.

  • Epitope
    HeLa and Jurkat lysates; Human endometrial carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
    • Tissue specificity
      Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
    • Sequence similarities
      Belongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.
    • Domain
      The PDZ-binding motif mediates interaction with GOPC.
      Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
      The FZ domain is involved in binding with Wnt ligands.
    • Post-translational
      modifications
      Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
    • Cellular localization
      Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Frizzled 8 seven transmembrane spanning receptor antibody
      • frizzled 8, seven transmembrane spanning receptor antibody
      • Frizzled family receptor 8 antibody
      • frizzled homolog 8 (Drosophila) antibody
      • Frizzled homolog 8 antibody
      • Frizzled-8 antibody
      • FZ 8 antibody
      • Fz-8 antibody
      • FZ8 antibody
      • FZD 8 antibody
      • FZD8 antibody
      • FZD8_HUMAN antibody
      • hFZ 8 antibody
      • hFz8 antibody
      • HGNC4046 antibody
      • Homolog of Drosophila Frizzled 8 antibody
      see all

    Images

    • All lanes : Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 73 kDa



      Primary antibody diluted with 1% BSA.

    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Frizzled 8 with ab155093 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155093 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab155093 showing +ve staining.

    References

    ab155093 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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