Anti-Frizzled 9 antibody (ab61430)
Key features and details
- Rabbit polyclonal to Frizzled 9
- Suitable for: IHC-P, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Frizzled 9 antibody
See all Frizzled 9 primary antibodies -
Description
Rabbit polyclonal to Frizzled 9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (Human Frizzled 9) conjugated to KLH
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.70
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 23251385
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 23251385 |
Target
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Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. -
Tissue specificity
Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Domain
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8326 Human
- Entrez Gene: 14371 Mouse
- Entrez Gene: 266608 Rat
- Omim: 601766 Human
- SwissProt: O00144 Human
- SwissProt: Q9R216 Mouse
- Unigene: 647029 Human
- Unigene: 6256 Mouse
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Alternative names
- CD 349 antibody
- CD 349 antigen antibody
- CD349 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab61430 has been referenced in 2 publications.
- Hachim MY et al. M1 Polarization Markers Are Upregulated in Basal-Like Breast Cancer Molecular Subtype and Associated With Favorable Patient Outcome. Front Immunol 11:560074 (2020). PubMed: 33304345
- Gonzalez P et al. Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats. PLoS One 7:e50793 (2012). IHC-P ; Rat . PubMed: 23251385