Anti-Frizzled 9 antibody [EPR4011] (ab108628)

Overview

  • Product nameAnti-Frizzled 9 antibody [EPR4011]
    See all Frizzled 9 primary antibodies
  • Description
    Rabbit monoclonal [EPR4011] to Frizzled 9
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Frizzled 9.

  • Positive control
    • Human seminoma, fetal kidney and fetal testis lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 64 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
    • Tissue specificityExpressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney.
    • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.
    • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
      The FZ domain is involved in binding with Wnt ligands.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 349 antibody
      • CD 349 antigen antibody
      • CD349 antibody
      • Frizzled Drosophila homolog of 3 antibody
      • Frizzled homolog 9 (Drosophila) antibody
      • Frizzled homolog 9 antibody
      • Frizzled-9 antibody
      • Fz 9 antibody
      • Fz-9 antibody
      • FZD 3 antibody
      • Fzd 9 antibody
      • FZD3 antibody
      • FZD3-PEN antibody
      • FZD9 antibody
      • FZD9_HUMAN antibody
      • FzE6 antibody
      • hFz9 antibody
      • mfz9 antibody
      • Xfz9 antibody
      see all

    Anti-Frizzled 9 antibody [EPR4011] images

    • All lanes : Anti-Frizzled 9 antibody [EPR4011] (ab108628) at 1/1000 dilution

      Lane 1 : Human Seminoma lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal testis lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 64 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)

    References for Anti-Frizzled 9 antibody [EPR4011] (ab108628)

    ab108628 has not yet been referenced specifically in any publications.

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