Anti-Frizzled homolog 1 antibody (ab117305)

Overview

  • Product nameAnti-Frizzled homolog 1 antibody
    See all Frizzled homolog 1 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled homolog 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Frizzled homolog 1.

  • Positive control
    • This antibody gave a positive signal in the following Human tissue lysates: Heart; Placenta; Lung; Kidney; Pancreas.

Properties

Applications

Our Abpromise guarantee covers the use of ab117305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).

Target

  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B.
  • Tissue specificityExpressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationMembrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chfz-1 antibody
    • Dfrizzled-1 antibody
    • DKFZp564G072 antibody
    • FLJ95923 antibody
    • Frizzled 1 antibody
    • Frizzled homolog 1 (Drosophila) antibody
    • Frizzled homolog 1 antibody
    • Frizzled-1 antibody
    • Fz receptor 1 antibody
    • Fz-1 antibody
    • Fz1 antibody
    • FZD1 antibody
    • FZD1_HUMAN antibody
    • FzE1 antibody
    • hFz1 antibody
    • Rfz1 antibody
    • Wnt receptor antibody
    • Xfrizzled-1 antibody
    see all

Anti-Frizzled homolog 1 antibody images

  • All lanes : Anti-Frizzled homolog 1 antibody (ab117305) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : Human kidney tissue lysate - total protein (ab30203)
    Lane 5 : Human pancreas tissue lysate - total protein (ab29816)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 40 kDa,47 kDa,88 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes

References for Anti-Frizzled homolog 1 antibody (ab117305)

ab117305 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab117305.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"