Anti-Frizzled homolog 1 antibody (ab71342)

Overview

  • Product nameAnti-Frizzled homolog 1 antibody
    See all Frizzled homolog 1 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled homolog 1
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the center of human Frizzled homolog 1, conjugated to KLH

  • Positive control
    • Hela cells lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ELISA 1/1000.
WB 1/50 - 1/100. Predicted molecular weight: 71 kDa.

Target

  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B.
  • Tissue specificityExpressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationMembrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chfz-1 antibody
    • Dfrizzled-1 antibody
    • DKFZp564G072 antibody
    • FLJ95923 antibody
    • Frizzled 1 antibody
    • Frizzled homolog 1 (Drosophila) antibody
    • Frizzled homolog 1 antibody
    • Frizzled-1 antibody
    • Fz receptor 1 antibody
    • Fz-1 antibody
    • Fz1 antibody
    • FZD1 antibody
    • FZD1_HUMAN antibody
    • FzE1 antibody
    • hFz1 antibody
    • Rfz1 antibody
    • Wnt receptor antibody
    • Xfrizzled-1 antibody
    see all

Anti-Frizzled homolog 1 antibody images

  • Anti-Frizzled homolog 1 antibody (ab71342) at 1/60 dilution + Hela cell line lysate at 35 µg

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 60 kDa,68 kDa,88 kDa. We are unsure as to the identity of these extra bands.
  • ab71342 at 1/50 dilution staining Frizzled homolog 1 in human prostate carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-Frizzled homolog 1 antibody (ab71342)

ab71342 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lung)
Loading amount 35 µg
Specification lung
Gel Running Conditions Reduced Denaturing (10 % SDS-Page gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 19 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"