Overview

  • Product name
  • Description
    Rabbit polyclonal to FRK
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of Human FRK

  • Positive control
    • Human breast carcinoma tissue, HUVEC cells and extracts from Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab64914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
  • Tissue specificity
    Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta cell Src homology tyrosine kinase antibody
    • BSK antibody
    • BSK/IYK antibody
    • C85044 antibody
    • Frk antibody
    • Frk fyn related kinase antibody
    • FRK_HUMAN antibody
    • FYN related kinase antibody
    • FYN-related kinase antibody
    • GASK antibody
    • Gastrointestinal associated kinase antibody
    • Gastrointestinal associated tyrosine kinase antibody
    • GTK antibody
    • GTK, mouse, homolog of antibody
    • Intestine tyrosine kinase antibody
    • Nuclear tyrosine protein kinase RAK antibody
    • Protein-tyrosine kinase 5 antibody
    • PTK5 antibody
    • PTK5 protein tyrosine kinase 5 antibody
    • RAK antibody
    • Tyrosine protein kinase FRK antibody
    • Tyrosine-protein kinase FRK antibody
    see all

Images

  • ab64914, at a 1/50 dilution, staining Human FRK in paraffin embedded breast carcinoma tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide.
  • All lanes : Anti-FRK antibody (ab64914) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • ab64914, at a 1/500 dilution, staining Human FRK in HUVEC cells, by Immunofluorescence, with (+) or without (-) immunising peptide.

References

ab64914 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

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Verified customer

Submitted Jun 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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