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Rabbit polyclonal to FRMD1
antigen sequence: RYFEPHSYFP QWIITKRGID YILRHMPTLH RERQGLSPKE AMLCFIQEAC RLEDVPVHFF RLHKDKKEGR PTVILGLALR GVHIYQGKKL EI, corresponding to amino acids 205-296 of Human FRMD1.
- Human bone marrow tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/250 - 1/500.
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 FERM domain.
Information by UniProt
- bA164L23.1 antibody
- DKFZp434O0117 antibody
- FERM domain containing 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-FRMD1 antibody (ab122109)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (actin filaments). Recommended concentration of ab122109 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FRMD1 antibody (ab122109)
All lanes : Anti-FRMD1 antibody (ab122109) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD1 antibody (ab122109)
ab122109, at 1/20 staining FRMD1 in paraffin embedded Human bone marrow by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"