Overview

  • Product name
    Anti-FRMD4A antibody
  • Description
    Rabbit polyclonal to FRMD4A
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: EGAHDKGAGR AAVSDELRQW YQRSTASHKE HSRLSHTSST SSDSGSQYST SSQSTFVAHS RVTRMPQMCK ATSA, corresponding to amino acids 912-985 of Human FRMD4A.

  • Positive control
    • Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and golgi apparatus. Recommended concentration of ab122475 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human testis tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Fan Y  et al. Increased expression of FERM domain-containing 4A protein is closely associated with the development of rectal cancer. Exp Ther Med 11:421-426 (2016). Read more (PubMed: 26893625) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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