Overview

  • Product name
    Anti-FRMD4B antibody
  • Description
    Rabbit polyclonal to FRMD4B
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 899-1009 of Human FRMD4B.

  • Positive control
    • Human cerebellum tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Member of GRP1 signaling complexes that are acutely recruited to plasma membrane ruffles in response to insulin receptor signaling. May function as a scaffolding protein.
  • Sequence similarities
    Contains 1 FERM domain.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6030440G05Rik antibody
    • FERM domain-containing protein 4B antibody
    • FRM4B_HUMAN antibody
    • Frmd4b antibody
    • GRP1-binding protein GRSP1 antibody
    • GRSP1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus and centrosome. Recommended concentration of ab121310 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121310 at 1/200 dilution staining FRMD4B in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry.

References

ab121310 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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