Anti-FRMD5 antibody (ab118737)

Overview

  • Product nameAnti-FRMD5 antibody
  • Description
    Rabbit polyclonal to FRMD5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 164-213 (FPKHSEKLEK KIAEIHKTEL SGQTPATSEL NFLRKAQTLE TYGVDPHPCK) of Rat FRMD5 (XP_230513).

  • Positive control
    • Rat Muscle lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-FRMD5 antibody images

  • Anti-FRMD5 antibody (ab118737) at 1 µg/ml + Rat Muscle lysate at 10 µg

    Predicted band size : 65 kDa

References for Anti-FRMD5 antibody (ab118737)

ab118737 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview for ab118737. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species (mouse), we would like to follow up on thi...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (cerebellum)
Loading amount 100 µg
Specification cerebellum
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Fuzi Jin

Verified customer

Submitted Nov 28 2012

Thank you for your phone call.
I am sorry to hear that the antibody did not work in mouse samples. Unfortunately, we have not yet tested mouse samples and thus do not know if and how well the antibody would work.

I am sending you the disco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"