Overview

  • Product name
  • Description
    Rabbit polyclonal to FRS2
  • Tested applications
    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 487-505 of Human FRS2alpha.

  • Positive control
    • Rat PC-12 and human HepG2 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. Use 10µg to 20ug using 3 mg RIPA lysate of mouse NIH/3T3 cells.
WB 1/1000. Detects a band of approximately 92, 95 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
  • Tissue specificity
    Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
  • Sequence similarities
    Contains 1 IRS-type PTB domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues upon stimulation by NGF.
    Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.
  • Cellular localization
    Endomembrane system. Cytoplasmic, membrane-bound.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGFR signaling adaptor SNT antibody
    • FGFR signalling adaptor antibody
    • FGFR substrate 2 antibody
    • FGFR-signaling adaptor SNT antibody
    • Fibroblast growth factor receptor substrate 2 antibody
    • FRS 2 antibody
    • FRS2 antibody
    • FRS2_HUMAN antibody
    • FRS2A antibody
    • FRS2alpha antibody
    • SNT 1 antibody
    • SNT antibody
    • SNT-1 antibody
    • SNT1 antibody
    • Suc 1 Associated Neurotrophic Factor Target antibody
    • Suc1 associated neurotrophic factor target 1 antibody
    • Suc1-associated neurotrophic factor target 1 antibody
    see all

Anti-FRS2 antibody images

  • Immunohistochemical analysis of murine aorta tissue, staining FRS2 with ab10425.

    Tissue was fixed with paraformaldehyde and blocked with 10% BSA and horse serum; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in 10% BSA and horse serum) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-FRS2 antibody (ab10425)

This product has been referenced in:

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (aorta cross section)
Specification
aorta cross section
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
Username

Abcam user community

Verified customer

Submitted Jan 03 2013

Thank you for your patience.

FRS2 is known to show a doublet around 92-95 kDa, as is reported in this paper: http://www.jbc.org/content/274/16/11321.full

The monomer form of FRS2 has a predicted molecular weight of 57 kDa, but the p...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry to learn that you are having difficulty getting the correct signal with ab10425. As we d...

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Thank you for providing your protocol details and an image of your blot. The originator of this antibody was able to provide the following information regarding the Western blot conditions that were originally used. Control: Human HepG2 cell lysate...

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Thank you for your enquiry. We don't produce ab10425 in-house, it is sourced from outside Abcam. I can't reveal the name of the originator, but I can tell you that we do not obtain the antibody from Santa Cruz. If you have any more questions, please do...

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