Overview

  • Product nameAnti-Fructose 6 Phosphate Kinase antibody
    See all Fructose 6 Phosphate Kinase primary antibodies
  • Description
    Mouse monoclonal to Fructose 6 Phosphate Kinase
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus Monkey
  • Immunogen

    Recombinant fragment: AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV , corresponding to amino acids 681-780 of Human Fructose 6 Phosphate Kinase (NP_000280) with a 26kDa tag.

  • Positive control
    • K-562 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 21482559
ELISA Use at an assay dependent dilution.

Target

  • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
  • Involvement in diseaseDefects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.
  • Sequence similaritiesBelongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6 Phosphofructokinase Muscle Type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase muscle type antibody
    • EC 2.7.1.1 antibody
    • EC 2.7.1.11 antibody
    • GSD7 antibody
    • K6PF_HUMAN antibody
    • MGC8699 antibody
    • muscle type antibody
    • PFK, muscle type antibody
    • PFK-A antibody
    • PFKA antibody
    • PFKL antibody
    • PFKM antibody
    • PFKP antibody
    • PFKX antibody
    • Phosphofructo 1 Kinase Isozyme A antibody
    • Phosphofructo-1-kinase isozyme A antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase M antibody
    • Phosphofructokinase, muscle antibody
    • Phosphofructokinase, muscle type antibody
    • Phosphofructokinase, polypeptide X antibody
    • Phosphofructokinase-M antibody
    • Phosphohexokinase antibody
    see all

Anti-Fructose 6 Phosphate Kinase antibody images

  • Anti-Fructose 6 Phosphate Kinase antibody (ab77159) at 5 µg/ml + Recombinant tagged human Fructose 6 Phosphate Kinase fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 85 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-Fructose 6 Phosphate Kinase antibody (ab77159) at 5 µg/ml + K-562 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 85 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

References for Anti-Fructose 6 Phosphate Kinase antibody (ab77159)

This product has been referenced in:
  • Hong M  et al. Cardiac ATP-sensitive K+ channel associates with the glycolytic enzyme complex. FASEB J 25:2456-67 (2011). WB, ICC/IF ; Rat . Read more (PubMed: 21482559) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"