Recombinant
RabMAb

Anti-Fructose 6 Phosphate Kinase antibody [EPR10734(B)] (ab154804)

Overview

  • Product name
    Anti-Fructose 6 Phosphate Kinase antibody [EPR10734(B)]
    See all Fructose 6 Phosphate Kinase primary antibodies
  • Description
    Rabbit monoclonal [EPR10734(B)] to Fructose 6 Phosphate Kinase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human Fructose 6 Phosphate Kinase. The exact sequence is proprietary.
    (Peptide available as ab207505)

  • Positive control
    • Fetal brain, fetal heart, Ramos, SHSY5Y and HeLa lysates; HeLa and SHSY5Y cells; Rat skeletal; Mouse skeletal; Pig heart lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
ICC/IF 1/300.

For unpurified use at 1: 50 - 1:100.

Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Pathway
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
  • Involvement in disease
    Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.
  • Sequence similarities
    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6 Phosphofructokinase Muscle Type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase muscle type antibody
    • EC 2.7.1.1 antibody
    • EC 2.7.1.11 antibody
    • GSD7 antibody
    • K6PF_HUMAN antibody
    • MGC8699 antibody
    • muscle type antibody
    • PFK, muscle type antibody
    • PFK-A antibody
    • PFKA antibody
    • PFKL antibody
    • PFKM antibody
    • PFKP antibody
    • PFKX antibody
    • Phosphofructo 1 Kinase Isozyme A antibody
    • Phosphofructo-1-kinase isozyme A antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase M antibody
    • Phosphofructokinase, muscle antibody
    • Phosphofructokinase, muscle type antibody
    • Phosphofructokinase, polypeptide X antibody
    • Phosphofructokinase-M antibody
    • Phosphohexokinase antibody
    see all

Images

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fructose 6 Phosphate Kinase with purified ab154804 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Fructose 6 Phosphate Kinase with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Fructose 6 Phosphate Kinase antibody [EPR10734(B)] (ab154804) at 1/10000 dilution (purified) + Pig heart lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • All lanes : Anti-Fructose 6 Phosphate Kinase antibody [EPR10734(B)] (ab154804) at 1/2000 dilution (purified)

    Lane 1 : Rat skeletal muscle lysates
    Lane 2 : Mouse skeletal muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Anti-Fructose 6 Phosphate Kinase antibody [EPR10734(B)] (ab154804) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 85 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Western blot analysis of Pig skeletal muscle tissue lysate using ab154804 (1:2000 in 1x PBS + 0.1% Tween-20 for 1 hour at 22°C) to label Fructose 6 Phosphate Kinase. A IRDye® 680-conjugated Donkey anti-rabbit IgG (1:40000) was used as the secondary antibody. The image shows increasing amounts of loaded protein for antibody optimization. The far left lane is the molecular weight standard.

    See Abreview

  • All lanes : Anti-Fructose 6 Phosphate Kinase antibody [EPR10734(B)] (ab154804) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal heart lysate
    Lane 3 : Ramos lysate
    Lane 4 : SHSY5Y lysate
    Lane 5 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 85 kDa

  • Immunofluorescence analysis of HeLa cells labeling Fructose 6 Phosphate Kinase with ab154804 at 1/50 dilution.
  • Flow cytometric analysis of permeabilized SHSY5Y cells labeling Fructose 6 Phosphate Kinase with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).

References

This product has been referenced in:
  • Boutant M  et al. Mfn2 is critical for brown adipose tissue thermogenic function. EMBO J 36:1543-1558 (2017). Read more (PubMed: 28348166) »
  • Bruns DR  et al. Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction. Am J Physiol Lung Cell Mol Physiol 308:L158-67 (2015). Read more (PubMed: 25416385) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
6 µg
Gel Running Conditions
Reduced Denaturing (10% 37.5:1)
Sample
Pig Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Eric England

Verified customer

Submitted Jul 31 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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