Overview

  • Product nameFructose Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    In Abcam's Fructose Assay Kit, free fructose is enzymatically converted to ß-glucose, which is then specifically converted to a product that reacts with OxiRed Probe to generate color (?=570nm) and fluorescence (Ex/Em=535/587nm). The kit provides a rapid, simple, sensitive, and reliable method suitable for high throughput assayof D-fructose.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Enzyme Mix (lyophilized) Green 1 vial
    Fructose Assay Buffer WM 1 x 25ml
    Fructose Converting Enzyme (Lyophilised) Purple 1 vial
    Fructose Standard (100mM) Yellow 1 x 100µl
    OxiRed Probe in DMSO Red 1 x 200µl
  • Research areas
  • RelevanceFructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.
  • Alternative names
    • Beta Levulose
    • D arabino Hexulose
    • Fruit sugar
    • Laevulose
    • Levulose
    see all

Applications

Our Abpromise guarantee covers the use of ab83380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Fructose Assay Kit images

  • Fructose levels banana, determined using the colorimetric (C) and the fluorometric (F) method (presented per mg of protein) (duplicates; +/- SD).

  • Fructose levels in mouse liver and banana, determined using the colorimetric method and presented per mg of protein (duplicates; +/- SD).

  • Titration of the fructose (background subtracted, duplicates; +/- SD).

  • Fluorometric Fructose Standard Curve performed following the ab83380 kit protocol.

Protocols

References for Fructose Assay Kit (ab83380)

ab83380 has not yet been referenced specifically in any publications.

Product Wall

Yes, samples made in RIPA buffer can be used, albeit, they need to be diluted in the respective assays buffers before use with the particular assays, so that the samples have all the components required for each reaction to occur.

Thank you for contacting us. The kit is currently priced at $435 and is for 100 tests. The protocol calls for 6 readings of standards, so if you take into account doing everything in duplicate (standards and samples) that would be 44 different samples....

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Thank you for your inquiry and for notifying us of the missing protocol.

The protocol has now been added to the datasheet and I also attached a copy for your customer to this email.

Please do not hesitate to contact us again with an...

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Thank you for your reply. We only have the full size kit available for purchase. However we guarantee all our products to work as stated on the datasheets, so we will be happy to help if the kit does not work as expected. Please let me kn...

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Thanks for your call yesterday and for your question about ab83380. I have been in contact with the lab, and I was told that the detection limit when the kit is used colorimetrically (570nm) is 1-10 nmols/well. If fluorometric methods (Ex/Em = ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"