Overview

  • Product name
  • Description
    Rabbit polyclonal to FRYL
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide: GSFAVIGSNL DMSEANYKLM ELNLEIRESL RMVQSYQLLA QAKPMGNMVS TGF, corresponding to a region within amino acids 2961-3013 of Human FRYL (NP_055845.1)

  • Positive control
    • HeLa and 293T Whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab95011 was affinity purified using an epitope specific to FRYL immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab95011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
  • Tissue specificity
    Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
  • Involvement in disease
    A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
  • Sequence similarities
    Belongs to the furry protein family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010313D22Rik antibody
    • 2310004H21Rik antibody
    • 2510002A14Rik antibody
    • 9030227G01Rik antibody
    • AF4P12 antibody
    • AI930088 antibody
    • ALL fused gene from chromosome 4p12 antibody
    • ALL1-fused gene from chromosome 4p12 protein antibody
    • DKFZp686E205 antibody
    • FLJ161772 antibody
    • FRY like antibody
    • FRYL antibody
    • FRYL_HUMAN antibody
    • Furry homolog like (Drosophila) antibody
    • Furry homolog like antibody
    • Furry like antibody
    • KIAA0826 antibody
    • mKIAA0826 antibody
    • Protein furry homolog-like antibody
    see all

Images

  • All lanes : Anti-FRYL antibody (ab95011) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 340 kDa


    Exposure time : 3 minutes
  • ab95011, at 1 µg/ml, detecting FRYL in HeLa whole cell lysate by WB following IP.
    Lane 1: IP with an antibody which recognizes an upstream epitope of FRYL
    Lane 2: ab95011 at 3 µg/mg of lysate
    Lane 3: Control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 10 seconds

References

ab95011 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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