- Datasheet
- Specific References (2)
- Protocols
Overview
-
Product nameAnti-FRYL antibody
See all FRYL primary antibodies -
DescriptionRabbit polyclonal to FRYL
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IPmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).
-
Positive control
- HeLa whole cell lysate, 293T cell lysate, Mouse NIH3T3 cell lysate,
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab95065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 340 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
-
FunctionPlays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
-
Tissue specificityWidely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
-
Involvement in diseaseA chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
-
Sequence similaritiesBelongs to the furry protein family.
- Information by UniProt
-
Database links
- Entrez Gene: 285527 Human
- Entrez Gene: 72313 Mouse
- Entrez Gene: 364144 Rat
- SwissProt: O94915 Human
- SwissProt: E9Q8I9 Mouse
- Unigene: 595553 Human
-
Alternative names
- 2010313D22Rik antibody
- 2310004H21Rik antibody
- 2510002A14Rik antibody
see all
Images
-
Western blot - Anti-FRYL antibody (ab95065)All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 340 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-FRYL antibody (ab95065)Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab95065 at 1 µg/ml.
Developed using the ECL technique with an exposure time of 1 seconds.
References
This product has been referenced in:
- Rippe C et al. Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. Sci Rep 7:1334 (2017). WB ; Mouse . Read more (PubMed: 28465505) »
- Peterson SM et al. Global gene expression analysis reveals dynamic and developmental stage-dependent enrichment of lead-induced neurological gene alterations. Environ Health Perspect 119:615-21 (2011). Read more (PubMed: 21147602) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"