Customer reviews and FAQs

Overview

  • Product name
    Anti-FRYL antibody
    See all FRYL primary antibodies
  • Description
    Rabbit polyclonal to FRYL
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).

  • Positive control
    • HeLa whole cell lysate, 293T cell lysate, Mouse NIH3T3 cell lysate,

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab95065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
  • Tissue specificity
    Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
  • Involvement in disease
    A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
  • Sequence similarities
    Belongs to the furry protein family.
  • Information by UniProt
  • Database links
    • Alternative names
      • 2010313D22Rik antibody
      • 2310004H21Rik antibody
      • 2510002A14Rik antibody
      • 9030227G01Rik antibody
      • AF4P12 antibody
      • AI930088 antibody
      • ALL fused gene from chromosome 4p12 antibody
      • ALL1-fused gene from chromosome 4p12 protein antibody
      • DKFZp686E205 antibody
      • FLJ161772 antibody
      • FRY like antibody
      • FRYL antibody
      • FRYL_HUMAN antibody
      • Furry homolog like (Drosophila) antibody
      • Furry homolog like antibody
      • Furry like antibody
      • KIAA0826 antibody
      • mKIAA0826 antibody
      • Protein furry homolog-like antibody
      see all

    Anti-FRYL antibody images

    • Western blot - FRYL antibody (ab95065)
      Western blot - FRYL antibody (ab95065)
      All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg
      Lane 5 : Mouse NIH3T3 cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 340 kDa


      Exposure time : 10 seconds
    • Immunoprecipitation - FRYL antibody (ab95065)
      Immunoprecipitation - FRYL antibody (ab95065)
      Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
      Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
      Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

      Subsequent Western blot detection used ab95065 at 1 µg/ml.

      Developed using the ECL technique with an exposure time of 1 seconds.

    References for Anti-FRYL antibody (ab95065)

    ab95065 has not yet been referenced specifically in any publications.

    Publishing research using ab95065? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab95065 Submit a question for ab95065

    1 Abreview or Q&A

    10
    Votes: Highest First

    I ordered 3 antibodies from Abcam on 1/3/12, and 2 of them worked quite well for western blot. However, Rb-anti-Fryl (ab95065) did not work in my western blots and I am wondering if you can provide troubleshooting suggestions or a refund.

    <...

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    I will be happy to help you with this.

    Our datasheet (http://www.abcam.com/fryl-antibody-ab95065.html) shows data similar to what your are seeing: many bands at lower molecular weights but also a single band at the expected size. These sampl...

    Read More

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