- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-FRYL antibody
See all FRYL primary antibodies - DescriptionRabbit polyclonal to FRYL
- Tested applicationsSuitable for: WB, IPmore details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Immunogen
Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).
- Positive control
- HeLa whole cell lysate, 293T cell lysate, Mouse NIH3T3 cell lysate,
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
Associated products
- Compatible Secondaries
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab95065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 340 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
- FunctionPlays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
- Tissue specificityWidely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
- Involvement in diseaseA chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
- Sequence similaritiesBelongs to the furry protein family.
- Information by UniProt
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Database links
- Entrez Gene: 285527 Human
- Entrez Gene: 72313 Mouse
- Entrez Gene: 364144 Rat
- SwissProt: O94915 Human
- SwissProt: E9Q8I9 Mouse
- Unigene: 595553 Human
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Alternative names
- 2010313D22Rik antibody
- 2310004H21Rik antibody
- 2510002A14Rik antibody
see all
Anti-FRYL antibody images
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Western blot - FRYL antibody (ab95065)All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 340 kDa
Exposure time : 10 seconds -
Immunoprecipitation - FRYL antibody (ab95065)Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab95065 at 1 µg/ml.
Developed using the ECL technique with an exposure time of 1 seconds.
References for Anti-FRYL antibody (ab95065)
ab95065 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"