Overview

  • Product nameAnti-FRYL antibody
    See all FRYL primary antibodies
  • Description
    Rabbit polyclonal to FRYL
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).

  • Positive control
    • HeLa whole cell lysate, 293T cell lysate, Mouse NIH3T3 cell lysate,

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab95065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionPlays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
  • Tissue specificityWidely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
  • Involvement in diseaseA chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
  • Sequence similaritiesBelongs to the furry protein family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010313D22Rik antibody
    • 2310004H21Rik antibody
    • 2510002A14Rik antibody
    • 9030227G01Rik antibody
    • AF4P12 antibody
    • AI930088 antibody
    • ALL fused gene from chromosome 4p12 antibody
    • ALL1-fused gene from chromosome 4p12 protein antibody
    • DKFZp686E205 antibody
    • FLJ161772 antibody
    • FRY like antibody
    • FRYL antibody
    • FRYL_HUMAN antibody
    • Furry homolog like (Drosophila) antibody
    • Furry homolog like antibody
    • Furry like antibody
    • KIAA0826 antibody
    • mKIAA0826 antibody
    • Protein furry homolog-like antibody
    see all

Anti-FRYL antibody images

  • All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 340 kDa


    Exposure time : 10 seconds
  • Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
    Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
    Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

    Subsequent Western blot detection used ab95065 at 1 µg/ml.

    Developed using the ECL technique with an exposure time of 1 seconds.

References for Anti-FRYL antibody (ab95065)

ab95065 has not yet been referenced specifically in any publications.

Product Wall

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Our datasheet (http://www.abcam.com/fryl-antibody-ab95065.html) shows data similar to what your are seeing: many bands at lower molecular weights but also a single band at the expected size. These sampl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"