Customer reviews and FAQs

Overview

  • Product name
    Anti-FRYL antibody
    See all FRYL primary antibodies
  • Description
    Rabbit polyclonal to FRYL
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).

  • Positive control
    • HeLa whole cell lysate, 293T cell lysate, Mouse NIH3T3 cell lysate,

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab95065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
  • Tissue specificity
    Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
  • Involvement in disease
    A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
  • Sequence similarities
    Belongs to the furry protein family.
  • Information by UniProt
  • Database links
    • Alternative names
      • 2010313D22Rik antibody
      • 2310004H21Rik antibody
      • 2510002A14Rik antibody
      • 9030227G01Rik antibody
      • AF4P12 antibody
      • AI930088 antibody
      • ALL fused gene from chromosome 4p12 antibody
      • ALL1-fused gene from chromosome 4p12 protein antibody
      • DKFZp686E205 antibody
      • FLJ161772 antibody
      • FRY like antibody
      • FRYL antibody
      • FRYL_HUMAN antibody
      • Furry homolog like (Drosophila) antibody
      • Furry homolog like antibody
      • Furry like antibody
      • KIAA0826 antibody
      • mKIAA0826 antibody
      • Protein furry homolog-like antibody
      see all

    Anti-FRYL antibody images

    • Western blot - FRYL antibody (ab95065)
      Western blot - FRYL antibody (ab95065)
      All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg
      Lane 5 : Mouse NIH3T3 cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 340 kDa


      Exposure time : 10 seconds
    • Immunoprecipitation - FRYL antibody (ab95065)
      Immunoprecipitation - FRYL antibody (ab95065)
      Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
      Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
      Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

      Subsequent Western blot detection used ab95065 at 1 µg/ml.

      Developed using the ECL technique with an exposure time of 1 seconds.

    References for Anti-FRYL antibody (ab95065)

    This product has been referenced in:
    • Peterson SM  et al. Global gene expression analysis reveals dynamic and developmental stage-dependent enrichment of lead-induced neurological gene alterations. Environ Health Perspect 119:615-21 (2011). Read more (PubMed: 21147602) »

    See 1 Publication for this product

    Publishing research using ab95065? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab95065 Submit a question for ab95065

    1 Abreview or Q&A

    10
    Votes: Highest First

    I ordered 3 antibodies from Abcam on 1/3/12, and 2 of them worked quite well for western blot. However, Rb-anti-Fryl (ab95065) did not work in my western blots and I am wondering if you can provide troubleshooting suggestions or a refund.

    <...

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    I will be happy to help you with this.

    Our datasheet (http://www.abcam.com/fryl-antibody-ab95065.html) shows data similar to what your are seeing: many bands at lower molecular weights but also a single band at the expected size. These sampl...

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