Overview

  • Product name
  • Description
    Rabbit polyclonal to FSCB
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 721-807 (ADLLLTEEFP IGEASAEVSP PPSEQTPEDE ALVENVSTEF QSPQVAGIPA VKLGSVVLEG EAKFEEVSKI NSVLKDLSNT NDGQAPT) of Human FSCB (Q5H9T9).

  • Positive control
    • Human Liver and Tonsil lysates; Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    May be involved in the later stages of fibrous sheath biogenesis. Binds calcium.
  • Cellular localization
    Cell projection > cilium > flagellum. Localizes to cortex of the fibrous sheath including the surface of the longitudinal columns and ribs of the principal piece of sperm flagella.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf155 antibody
    • Fibrous sheath CABYR-binding protein antibody
    • Fscb antibody
    • FSCB_HUMAN antibody

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121201 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-FSCB antibody (ab121201) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121201 at 1/300 dilution staining FSCB in Paraffin-emebedded Human testis tissue by Immunohistochemistry.

References

ab121201 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121201.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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