Anti-FSH Receptor antibody (ab113421)

Overview

  • Product nameAnti-FSH Receptor antibody
    See all FSH Receptor primary antibodies
  • Description
    Rabbit polyclonal to FSH Receptor
  • Specificityab113421 detects endogenous levels of total FSH Receptor protein. Immunogenic peptide has only 57% homology with the corresponding region in mouse.
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids of Human FSH Receptor.

  • Positive control
    • Human Ovary tissue; MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab113421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • FunctionReceptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificitySertoli cells and ovarian granulosa cells.
  • Involvement in diseaseDefects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1) [MIM:233300]; also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
    Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS) [MIM:608115]. OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications
    N-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Follicle stimulating hormone receptor antibody
    • Follicle stimulating hormone receptor isoform 1 antibody
    • Follicle-stimulating hormone receptor antibody
    • Follitropin receptor antibody
    • FSH receptor antibody
    • FSH-R antibody
    • Fshr antibody
    • FSHR_HUMAN antibody
    • FSHRO antibody
    • LGR1 antibody
    • MGC141667 antibody
    • MGC141668 antibody
    • ODG1 antibody
    • ovarian dysgenesis 1 antibody
    see all

Anti-FSH Receptor antibody images

  • ab113421 at 10 µg/ml staining FSH Receptor in Human ovary by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • Immunofluorescence analysis of MCF7 cells, using ab113421 at 1/100 dilution. The image on the right is treated with the synthesized peptide.

References for Anti-FSH Receptor antibody (ab113421)

ab113421 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Syrian Hamster Tissue lysate - whole (Ovary)
Loading amount 80 µg
Specification Ovary
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. SK Roy

Verified customer

Submitted Feb 10 2012

DISCOUNT CODE: *** Expiration date: April 9, 2012 I am very pleased to hear you would like to accept our offer and test ab113421 in hamster samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, pleas...

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Thank you for contacting us. The anti-FSH receprot antibody ab11321 was raised against an immunogen located between residues 280 and 330 of human FSHR.  Refering to the SwissProt reference P23945, this entire region is located within the N-terminal ext...

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Thank you for considering the testing discount we discussed with your mouse IHC study. Unfortunately as we carry over 70,000 products, it is unfeasible for us to keep small sample sizes of our products. I am therefore unable to offer you a free sample ...

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Thank you for contacting us with your queries. Unfortunately I would not advise using ab113421 with mouse samples as the immunogenic peptide used in producing this antibody only shares 57% homology with the corresponding region in mouse. This h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"