Overview

  • Product name
    Anti-FSH Receptor antibody
    See all FSH Receptor primary antibodies
  • Description
    Rabbit polyclonal to FSH Receptor
  • Specificity
    BLAST analysis of the peptide immunogen showed no homology with other human proteins.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig, Monkey, Gorilla, Opossum, Common marmoset, House sparrow, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to 18 amino acids from the N-terminal extracellular domain of Human FSH Receptor.

  • Positive control
    • Human ovary tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab150557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 - 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificity
    Sertoli cells and ovarian granulosa cells.
  • Involvement in disease
    Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1) [MIM:233300]; also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
    Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS) [MIM:608115]. OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications
    N-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Follicle stimulating hormone receptor antibody
    • Follicle stimulating hormone receptor isoform 1 antibody
    • Follicle-stimulating hormone receptor antibody
    • Follitropin receptor antibody
    • FSH receptor antibody
    • FSH-R antibody
    • Fshr antibody
    • FSHR_HUMAN antibody
    • FSHRO antibody
    • LGR1 antibody
    • MGC141667 antibody
    • MGC141668 antibody
    • ODG1 antibody
    • ovarian dysgenesis 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovary tissue, labeling FSH Receptor with ab150557 at 8 µg/ml.

References

This product has been referenced in:
  • Feng Z  et al. Expression of hypothalamic-pituitary-gonadal axis-related hormone receptors in low-grade serous ovarian cancer (LGSC). J Ovarian Res 10:7 (2017). Read more (PubMed: 28122595) »
  • Feng Z  et al. A clinically applicable molecular classification for high-grade serous ovarian cancer based on hormone receptor expression. Sci Rep 6:25408 (2016). IHC . Read more (PubMed: 27139372) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (endometrium, myomerium)
Permeabilization
No
Specification
endometrium, myomerium
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
background buster from innovex as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric pH6 20min 100C
Sample
Mouse Tissue sections (testis)
Specification
testis
Permeabilization
Yes - proclin 300
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up