Overview

  • Product name
  • Description
    Goat polyclonal to FSTL1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide: TAEKTKRVSTKEI, corresponding to amino acids 296-308 of Human FSTL1.

  • Positive control
    • Human Placenta and Ovary lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human FSTL1 peptide (ab23213). The observed molecular weight corresponds to earlier findings in literature (Tanaka et al, Int Immunol. 1998 Sep;10(9):1305-14.

Target

Anti-FSTL1 antibody images

References for Anti-FSTL1 antibody (ab11805)

This product has been referenced in:
  • Sundaram GM  et al. 'See-saw' expression of microRNA-198 and FSTL1 from a single transcript in wound healing. Nature 495:103-6 (2013). Read more (PubMed: 23395958) »
  • Slany A  et al. Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B. J Proteome Res 9:6-21 (2010). WB ; Human . Read more (PubMed: 19678649) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

Unfortunately it won't be possible to send the replacement to your collaborator.

What about ab107431?This rabbit polyclonal antibody is directed against the C-terminal part of Human FSTL1 and the immunogen...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (mesenchymal stem cells)
Loading amount
8 µg
Specification
mesenchymal stem cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

Submitted May 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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