Overview

  • Product name
  • Description
    Goat polyclonal to FSTL1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide: TAEKTKRVSTKEI, corresponding to amino acids 296-308 of Human FSTL1.

  • Positive control
    • Human Placenta and Ovary lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human FSTL1 peptide (ab23213). The observed molecular weight corresponds to earlier findings in literature (Tanaka et al, Int Immunol. 1998 Sep;10(9):1305-14.

Target

Images

References

This product has been referenced in:
  • Mouillet JF  et al. The expression and post-transcriptional regulation of FSTL1 transcripts in placental trophoblasts. Placenta 36:1231-8 (2015). Read more (PubMed: 26386648) »
  • Sundaram GM  et al. 'See-saw' expression of microRNA-198 and FSTL1 from a single transcript in wound healing. Nature 495:103-6 (2013). Read more (PubMed: 23395958) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

Unfortunately it won't be possible to send the replacement to your collaborator.

What about ab107431?This rabbit polyclonal antibody is directed against the C-terminal part of Human FSTL1 and the immunogen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (mesenchymal stem cells)
Loading amount
8 µg
Specification
mesenchymal stem cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

Submitted May 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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