Anti-FSTL1/FRP antibody (ab232777)
Key features and details
- Rabbit polyclonal to FSTL1/FRP
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-FSTL1/FRP antibody
See all FSTL1/FRP primary antibodies -
Description
Rabbit polyclonal to FSTL1/FRP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Macaque monkey, Orangutan -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human FSTL1/FRP aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q12841 -
Positive control
- WB: Recombinant human FSTL1/FRP protein; Pig liver and heart tissue lysates. IHC-P: Human prostate, breast cancer, liver, kidney and stomach tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin. -
Tissue specificity
Overexpressed in synovial tissues from rheumatoid arthritis. -
Sequence similarities
Contains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 534482 Cow
- Entrez Gene: 11167 Human
- Entrez Gene: 14314 Mouse
- Entrez Gene: 100172767 Orangutan
- Entrez Gene: 79210 Rat
- Omim: 605547 Human
- SwissProt: Q58D84 Cow
- SwissProt: Q12841 Human
see all -
Alternative names
- Anti FSTL1 antibody
- FLJ50214 antibody
- FLJ52277 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human prostate tissue stained for FSTL1/FRP using ab232777 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for FSTL1/FRP using ab232777 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for FSTL1/FRP using ab232777 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for FSTL1/FRP using ab232777 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for FSTL1/FRP using ab232777 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-FSTL1/FRP antibody (ab232777) at 1 µg/ml + Recombinant human FSTL1/FRP protein
Developed using the ECL technique.
Predicted band size: 35 kDa -
Anti-FSTL1/FRP antibody (ab232777) at 1 µg/ml + Pig liver tissue lysate
Developed using the ECL technique.
Predicted band size: 35 kDa -
Anti-FSTL1/FRP antibody (ab232777) at 1 µg/ml + Pig heart tissue lysate
Developed using the ECL technique.
Predicted band size: 35 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab232777 has been referenced in 1 publication.
- Wang Q et al. MicroRNA-124-3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus-like cells via targeting FSTL1. Exp Ther Med 22:725 (2021). PubMed: 34007334