Anti-FSTL3 antibody - C-terminal (ab170680)

Overview

  • Product name
    Anti-FSTL3 antibody - C-terminal
    See all FSTL3 primary antibodies
  • Description
    Rabbit polyclonal to FSTL3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FSTL3 aa 228-258 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O95633

  • Positive control
    • CEM cell lysate, formalin fixed and paraffin embedded Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 28 kDa.

Target

  • Function
    Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.
  • Tissue specificity
    Expressed in a wide range of tissues.
  • Involvement in disease
    Note=A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1.
  • Sequence similarities
    Contains 2 follistatin-like domains.
    Contains 2 Kazal-like domains.
    Contains 1 TB (TGF-beta binding) domain.
  • Cellular localization
    Secreted and Nucleus. Although alternative initiation has been demonstrated and resulted in different localization, the major source of nuclear FSTL3 appears not to depend on translation initiation at Met-27 according to.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLRG antibody
    • follistatin-like 3 antibody
    • follistatin-like 3 glycoprotein antibody
    • Follistatin-like protein 3 antibody
    • Follistatin-related gene protein antibody
    • Follistatin-related protein 3 antibody
    • FSRP antibody
    • Fstl3 antibody
    • FSTL3_HUMAN antibody
    see all

Images

  • Anti-FSTL3 antibody - C-terminal (ab170680) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size: 28 kDa

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human testis tissue labeling FSTL3 with ab170680 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170680 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide)
Loading amount
20 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jeremiah Gaudet

Verified customer

Submitted Aug 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Specification
Placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Jeremiah Gaudet

Verified customer

Submitted Jul 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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