• Product nameAnti-FTO antibody
    See all FTO primary antibodies
  • Description
    Rabbit polyclonal to FTO
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    15 amino acid peptide from near the amino terminus of Human FTO.

  • Positive control
    • Human uterus tissue lysate Mouse brain tissue


Our Abpromise guarantee covers the use of ab106386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P Use a concentration of 2.5 µg/ml.


  • FunctionDioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
  • Tissue specificityUbiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
  • Involvement in diseaseDefects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
  • Sequence similaritiesBelongs to the fto family.
  • DomainThe 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AlkB homolog 9 antibody
    • ALKBH9 antibody
    • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
    • AW743446 antibody
    • Fat mass and obesity-associated protein antibody
    • FATSO, MOUSE, HOMOLOG OF antibody
    • Fto antibody
    • FTO_HUMAN antibody
    • GDFD antibody
    • KIAA1752 antibody
    • mKIAA1752 antibody
    • Protein fatso antibody
    see all

Anti-FTO antibody images

  • Lane 1 : Anti-FTO antibody (ab106386) at 1 µg/ml
    Lane 2 : Anti-FTO antibody (ab106386) at 2 µg/ml

    Lane 1 : Human uterus tissue lysate
    Lane 2 : Human uterus tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 58 kDa
  • Immunohistochemistry of FTO in Mouse brain tissue with ab106386 at 2.5 µg/ml.
  • Immunofluorescence of FTO in Mouse Brain cells using ab106386 at 20 ug/ml.

References for Anti-FTO antibody (ab106386)

ab106386 has not yet been referenced specifically in any publications.

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