• Product nameAnti-FTO antibody [EPR6895]
    See all FTO primary antibodies
  • Description
    Rabbit monoclonal [EPR6895] to FTO
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human FTO.

  • Positive control
    • Human Breast and kidney tissues; 293T, SH-SY5Y, Caco2 and BxPC3 lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab124892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionDioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
    • Tissue specificityUbiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
    • Involvement in diseaseDefects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
    • Sequence similaritiesBelongs to the fto family.
    • DomainThe 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AlkB homolog 9 antibody
      • ALKBH9 antibody
      • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
      • AW743446 antibody
      • Fat mass and obesity-associated protein antibody
      • FATSO, MOUSE, HOMOLOG OF antibody
      • Fto antibody
      • FTO_HUMAN antibody
      • GDFD antibody
      • KIAA1752 antibody
      • mKIAA1752 antibody
      • Protein fatso antibody
      see all

    Anti-FTO antibody [EPR6895] images

    • ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human Breast tissue by Immunohistochemistry.
    • All lanes : Anti-FTO antibody [EPR6895] (ab124892) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Caco2 lysate
      Lane 4 : BxPC3 lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
    • ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-FTO antibody [EPR6895] (ab124892)

    This product has been referenced in:
    • Mauer J  et al. Reversible methylation of m(6)Am in the 5' cap controls mRNA stability. Nature 541:371-375 (2017). WB ; Human . Read more (PubMed: 28002401) »
    • Tirumuru N  et al. N(6)-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression. Elife 5:N/A (2016). WB ; Human . Read more (PubMed: 27371828) »

    See all 5 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HepG2)
    Permeabilization Yes - 0.05% Triton
    Specification HepG2
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Paraformaldehyde

    Fange Liu

    Verified customer

    Submitted Mar 09 2017