The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 1 - 5 µg/mg of lysate.
WB: 1/5000 - 1/25000. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.
FtsJ methyltransferase domain-containing protein 2 antibody
FTSJD 2 antibody
Interferon-stimulated gene 95 kDa protein antibody
Uncharacterized protein KIAA0082 antibody
Western blot - Anti-FTSJD2 antibody (ab70386)
All lanes : Anti-FTSJD2 antibody (ab70386) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells transfected with a myc-FTSJD2 expression construct at 15 µg Lane 2 : Whole cell lysate 293T cells that were mock transfected at 60 µg Lane 3 : Whole cell lysate from G361 cells at 15 µg