Overview

  • Product nameAnti-FUBP1 antibody
    See all FUBP1 primary antibodies
  • Description
    Rabbit polyclonal to FUBP1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: KMVMIQDGPQ NTGADKPLRI TGDPYKVQQA KEMVLELIRD QGGFREVRNE , corresponding to amino acids 218-267 of Human FUBP1

  • Positive control
    • HepG2 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

Target

  • FunctionRegulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
  • Sequence similaritiesContains 4 KH domains.
  • Post-translational
    modifications
    Ubiquitinated. This targets the protein for proteasome-mediated degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA helicase V antibody
    • far upstream element (FUSE) binding protein 1 antibody
    • Far upstream element (FUSE) binding protein 4 antibody
    • Far upstream element binding protein 1 antibody
    • far upstream element binding protein antibody
    • Far upstream element-binding protein 1 antibody
    • FBP antibody
    • FUBP antibody
    • Fubp1 antibody
    • FUBP1_HUMAN antibody
    • Fubp4 antibody
    • FUSE binding protein 1 antibody
    • FUSE-binding protein 1 antibody
    • HDH V antibody
    see all

Anti-FUBP1 antibody images

  • Anti-FUBP1 antibody (ab66603) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + HepG cells lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)

References for Anti-FUBP1 antibody (ab66603)

ab66603 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"