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Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (YYAHYYQQQA QPPPAAPAGA PTTTQTNGQG DQQNPAPAGQ VDYTKAWEEY) of human FUBP1 (NP_003893).
Our Abpromise guarantee covers the use of ab83453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:1562500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling FUBP1 with ab83453 at 5.0µg/ml.
ab83453 has not yet been referenced specifically in any publications.