Overview

  • Product nameAnti-FUBP1 antibody
    See all FUBP1 primary antibodies
  • Description
    Rabbit polyclonal to FUBP1
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (YYAHYYQQQA QPPPAAPAGA PTTTQTNGQG DQQNPAPAGQ VDYTKAWEEY) of human FUBP1 (NP_003893).

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

Target

  • FunctionRegulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
  • Sequence similaritiesContains 4 KH domains.
  • Post-translational
    modifications
    Ubiquitinated. This targets the protein for proteasome-mediated degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA helicase V antibody
    • far upstream element (FUSE) binding protein 1 antibody
    • Far upstream element (FUSE) binding protein 4 antibody
    • Far upstream element binding protein 1 antibody
    • far upstream element binding protein antibody
    • Far upstream element-binding protein 1 antibody
    • FBP antibody
    • FUBP antibody
    • Fubp1 antibody
    • FUBP1_HUMAN antibody
    • Fubp4 antibody
    • FUSE binding protein 1 antibody
    • FUSE-binding protein 1 antibody
    • HDH V antibody
    see all

Anti-FUBP1 antibody images

  • Anti-FUBP1 antibody (ab83453) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling FUBP1 with ab83453 at 5.0µg/ml.

References for Anti-FUBP1 antibody (ab83453)

ab83453 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"