Overview

  • Product name
  • Description
    Rabbit polyclonal to FUBP3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 522 - 572 of Human FUBP3 (NP_003925.1)

  • Positive control
    • HeLa, 293T or NIH 3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab108222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
  • Tissue specificity
    Detected in a number of cell lines.
  • Sequence similarities
    Contains 4 KH domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Far upstream element (FUSE) binding protein 3 antibody
    • Far upstream element binding protein 3 antibody
    • Far upstream element-binding protein 3 antibody
    • FBP3 antibody
    • FLJ25229 antibody
    • FUBP 3 antibody
    • FUBP3 antibody
    • FUBP3_HUMAN antibody
    • FUSE binding protein 3 antibody
    • FUSE-binding protein 3 antibody
    • OTTHUMP00000022369 antibody
    see all

Images

  • All lanes : Anti-FUBP3 antibody (ab108222) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 62 kDa


    Exposure time : 3 minutes
  • Detection of FUBP3 by western blot of immunoprecipitate.
    ab108222 at 1 µg/ml staining FUBP3 in HeLa whole cell lysate immunoprecipitated using ab108222 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: chemiluminescence with an exposure time of 30 seconds.

References

ab108222 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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