Recombinant
RabMAb

Anti-FUBP3 antibody [EPR13174-61] (ab181122)

Overview

  • Product name
    Anti-FUBP3 antibody [EPR13174-61]
    See all FUBP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR13174-61] to FUBP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FUBP3 aa 400-550. The exact sequence is proprietary.
    Database link: Q96I24

  • Positive control
    • MCF7, U87-MG or HepG2 lysate, Human kidney clear cell carcinoma or colon tissue, HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.

WB 1/1000 - 1/20000. Detects a band of approximately 62 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Function
    May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
  • Tissue specificity
    Detected in a number of cell lines.
  • Sequence similarities
    Contains 4 KH domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Far upstream element (FUSE) binding protein 3 antibody
    • Far upstream element binding protein 3 antibody
    • Far upstream element-binding protein 3 antibody
    • FBP3 antibody
    • FLJ25229 antibody
    • FUBP 3 antibody
    • FUBP3 antibody
    • FUBP3_HUMAN antibody
    • FUSE binding protein 3 antibody
    • FUSE-binding protein 3 antibody
    • OTTHUMP00000022369 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling FUBP3 with ab181122 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

  • Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/5000 dilution + HepG2 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  • All lanes : Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/20000 dilution

    Lane 1 : MCF7 lysate
    Lane 2 : U87-MG lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

References

ab181122 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181122.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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