Recombinant
RabMAb

Anti-FUCA1 antibody [EPR12498] (ab181357)

Overview

  • Product name
    Anti-FUCA1 antibody [EPR12498]
    See all FUCA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12498] to FUCA1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FUCA1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P04066

  • Positive control
    • K562, Human fetal liver, and Human fetal kidney lysates, permeabilized K562 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab181357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.Can be blocked with FUCA1 peptide (ab208990).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
    • Involvement in disease
      Defects in FUCA1 are the cause of fucosidosis (FUCA1D) [MIM:230000]. FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas.
    • Sequence similarities
      Belongs to the glycosyl hydrolase 29 family.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha L fucosidase I antibody
      • Alpha L fucoside fucohydrolase antibody
      • Alpha-L-fucosidase 1 antibody
      • Alpha-L-fucosidase I antibody
      • Alpha-L-fucoside fucohydrolase 1 antibody
      • FUCA antibody
      • FUCA1 antibody
      • FUCO_HUMAN antibody
      • fucosidase, alpha-L, tissue antibody
      • fucosidase, alpha-L- 1, tissue antibody
      • Tissue alpha L fucosidase antibody
      • Tissue alpha-L-fucosidase antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized K562 cells using ab181357 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

    • All lanes : Anti-FUCA1 antibody [EPR12498] (ab181357) at 1/1000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : Human fetal liver lysates
      Lane 3 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 54 kDa

    References

    ab181357 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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