Overview

  • Product nameAnti-FUK antibody
    See all FUK primary antibodies
  • Description
    Rabbit polyclonal to FUK
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human FUK.

  • Positive control
    • Extracts from insulin-treated COS7 cells. NIH-3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+) , 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 118 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

Anti-FUK antibody images

  • All lanes : Anti-FUK antibody (ab69555) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells, treated with Insulin (0.01U/ml, 45mins)
    Lane 2 : Extracts from COS7 cells, treated with Insulin (0.01U/ml, 45mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 118 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of FUK expression on NIH-3T3 cells, using ab 69555 at 1/500 dilution, with and without the immunising peptide.

References for Anti-FUK antibody (ab69555)

ab69555 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"