Fumarase Human ELISA profiling Kit (ab157399)
- Product nameFumarase Human ELISA profiling KitSee all Fumarase kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 3 = 2.93%
- Sample typeCell Lysate, Tissue Lysate
- Assay typeSandwich (qualitative)
- Sensitivity= 0.409 µg/ml
- Range0 µg/ml - 125 µg/ml
Sample specific recovery Sample type Average % Range Serum 111 107% - 116% Cell culture media = 120 119% - 127% Extraction Buffer 119 119% - 124%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
Does not react withMouse, Rat
- Product overview
Abcam’s Fumarase (FH) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Fumarase protein in Human cell and tissue lysates.
The assay employs an antibody specific to FH protein coated onto well plate strips. Standards and samples are pipetted into the wells and FH present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-FH detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of FH bound to the plate well. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Fumarase (FH, Fumarate hydratase, P07954) is a 50 kDa enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate (EC 22.214.171.124). Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme, exists without the mitochondrial import sequence, is involved in the metabolism of cytoplasmic fumarate, a byproduct of the urea and amino acid metabolism.
Defects in fumarase (FH) are the cause of fumarase deficiency [MIM606812], also known as fumaricaciduria, which is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic/pyruvic academia. Additionally FH is a tumor suppressor gene; inactivating mutations in the FH gene are responsible for hereditary leiomyomataosis renal cell carcinoma (HLRCC) [MIM:150800], a disorder characterized by predisposition to cutaneous and uterine leiomyomas, and papillary type 2 renal cancer which occurs in about 20% of patients.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X FH Detector Antibody 1 x 1ml 10X HRP Label 1 x 1ml 20X Buffer 1 x 20ml Extraction Buffer 1 x 15ml FH Microplate 1 unit Hela Lysate Control (200ug) 1 x 200µg TMB Development Solution 1 x 12ml
- Fumarate hydratase, mitochondrial
Our Abpromise guarantee covers the use of ab157399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Fumarase Human ELISA profiling Kit images
A dilution series of Hela cell lysates (standard sample) in the working range of the assay.
Sample experiment showing FH levels in human liver homogenate (HLH), HepG2 cell lysates, HDFn cell lysates, MCF7 cell lysates and HeLa cell lysates, which is shown as a standard control sample. Cell lysates at varying concentrations within the working range of the assay were analyzed.
Sample experiment showing FH levels in human liver homogenate (HLH), HepG2 cell lysates, HDFn cell lysates, MCF7 cell lysates and HeLa cell lysates, which is shown as a standard control sample. The protein expression level in different samples were demonstrated relatively to Hela Standard
Figure 4. (Left) The FH Detector Antibody used in the ELISA kit immunocaptures FH from HepG2 Cell Lysate (lane 2), Human Liver Homogenate (lane 3), bovine Heart Homogenate (lane 4) and Rat Heart Homogenate (lane 5). Target has been confirmed by Mass Spectometric data. (Right) The antibody also works in immunocytochemistry and targets on Mitochondrial isozyme of FH.
Figure 3. (Left) The FH Capture Antibody used in the ELISA kit immunocaptures FH from Human Liver Homogenate (lane 2), bovine Heart Homogenate (lane 3) and HepG2 Cell Lysate (lane 4). Target has been confirmed by Mass Spectometric data. (Right) The antibody also works in immunocytochemistry and targets on Mitochondrial isozyme of FH.
References for Fumarase Human ELISA profiling Kit (ab157399)
ab157399 has not yet been referenced specifically in any publications.