MitoSciences (MS780)

Fumarase Specific Activity Assay Kit (ab110043)

Overview

  • Product nameFumarase Specific Activity Assay KitSee all Fumarase kits ...
  • Detection methodColorimetric
  • Assay typeEnzyme activity
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat, Cow, Human

    Does not react with

    Mouse
  • Product overview

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • Notes

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab110043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent dilution.

Fumarase Specific Activity Assay Kit images

  • Example data obtained using the Fumarase Specific Activity Assay Kit (ab110043).
  • Principle of a Sandwich ELISA.

Protocols

References for Fumarase Specific Activity Assay Kit (ab110043)

ab110043 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and for bringing this error to our attention.

The units should all me in ug, not mg.

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Thank you for contacting us.

I can confirm that ab110043 Fumarase Specific Activity Assay Kit does worki with Rat, Cow and Human samples. This product will not work with mouse.

I hope this information is helpful to you. Please...

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Thank you for contacting us.

The antibody used in this kit detects only human, rat and cow FH. It does not detect it in mouse samples. Therefore, we do not expect to see the activity from mouse samples in this immunocapture based activity as...

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I am still waiting for mouse reactivity data for the fumarase assay, ab110043. I did confirm, however, that the ATP synthase assay, ab109714, does come with 12 strips of 8 wells each, and not a standard solid microplate.

Thank you very much for your interest in our products.

I just sent you Proforma Invoice (quotation) #45550 for the 4 kits. This quote will be good until Sept 30th when the discount expires. When you're ready to place the order, just call us a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"