- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-FUNDC1 antibody - N-terminal
See all FUNDC1 primary antibodies -
DescriptionRabbit polyclonal to FUNDC1 - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide within Human FUNDC1 aa 78-108 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q8IVP5 -
Positive control
- NCI-H460 cell line lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab173226 is purified through a Protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab173226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 17 kDa. |
Target
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FunctionActs as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.
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Tissue specificityWidely expressed.
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Sequence similaritiesBelongs to the FUN14 family.
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DomainThe YXXL motif mediates the interaction with MAP1 LC3 family proteins MAP1LC3A, MAP1LC3B and GABARAP (PubMed:22267086).
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Post-translational
modificationsPhosphorylation at Tyr-18 by SRC inhibits activation of mitophagy. Following hypoxia, dephosphorylated at Tyr-18, leading to interaction with MAP1 LC3 family proteins and triggering mitophagy. -
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
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Database links
- Entrez Gene: 518258 Cow
- Entrez Gene: 139341 Human
- Entrez Gene: Mouse
- Entrez Gene: 72018 Mouse
- Entrez Gene: 363442 Rat
- Omim: 300871 Human
- SwissProt: F1N5S9 Cow
- SwissProt: Q8IVP5 Human
see all -
Alternative names
- FUN14 domain containing 1 antibody
- FUN14 domain containing protein 1 antibody
- FUN14 domain-containing protein 1 antibody
see all
Images
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Western blot - Anti-FUNDC1 antibody - N-terminal (ab173226)Anti-FUNDC1 antibody - N-terminal (ab173226) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg
Predicted band size: 17 kDa
Protocols
References
ab173226 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"