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Anti-Furin antibody (ab3467)

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Overview

Product name

Anti-Furin antibody
See all Furin products (5) ...

Description

Rabbit polyclonal to Furin

Specificity

Detects Furin from canine and mouse cells as well as transfected human Furin. This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.

Tested applications

ICC/IF, IF, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Dog, Human

Predicted to work with

Rat

Immunogen

Synthetic peptide: RGERTAFIKDQSAL, corresponding to amino acids 780-793 of Human Furin(Peptide available as ab4989.)

RGERTAFIKD QSAL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, 1 mg/ml BSA and 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Primary antibody notes

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Signal Transduction >> Metabolism >> Amino Acids

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Furin antibody (ab3467)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Furin antibody (ab3467) image (enlarge)

  • Western blot - Furin antibody (ab3467)Western blot - Furin antibody (ab3467) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 18174282)

  • ShowHide

    IF

     IF: Use at an assay depe...Read more →

    IF: Use at an assay dependent dilution.

  • IP

     IP: 1/100

    IP: 1/100

  • ShowHide1 Image

    WB

    No star rating (1 Abreview) WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).(Representing Furin in BSC-40 cells transfected with the human Furin gene. )

Target

Relevance

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

Cellular localization

Golgi apparatus; trans-Golgi network.

Alternative names

  • Dibasic processing enzyme antibody
  • PACE antibody
  • Paired basic amino acid residue cleaving enzyme antibody
  • FES upstream region antibody
  • FUR antibody
  • PCSK3 antibody
  • Proprotein convertase subtilisin/kexin type 3 antibody
  • SPC1 antibody
see all

Database links

Anti-Furin antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Furin antibody (ab3467)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Furin antibody (ab3467)

Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.

  Western blot - Furin antibody (ab3467)

Western blot - Furin antibody (ab3467)

All lanes : Anti-Furin antibody (ab3467) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 87 kDa
Observed band size : 63 kDa (why is the actual band size different from the predicted?)


Exposure time : 90 seconds

The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.

References for Anti-Furin antibody (ab3467)

This product has been referenced in:

  • Sumara Get al. Regulation of PKD by the MAPK p38delta in insulin secretion and glucose homeostasis. Cell 136:235-48 (2009). ICC/IF; Mouse.Read more (PubMed: 19135240) »
  • Huynh TTet al. Effect of ultraviolet radiation on the expression of pp38MAPK and furin in human keratinocyte-derived cell lines. Photodermatol Photoimmunol Photomed 25:20-9 (2009). WB; Human.Read more (PubMed: 19152512) »

See all 5 publications for this product

Publishing research using ab3467? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"