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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Overview
Product name
Anti-Furin antibody - Aminoterminal end
See all Furin products (5) ...
Description
Rabbit polyclonal to Furin - Aminoterminal end
Specificity
ab28547 recognizes subtilisin-like serine protease furin.
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide based on the amino end of mature human furin.
(Peptide available as ab41397.)
Positive control
Furin is ubiquitously expressed at low levels, but found in highest concentration in the liver and kidneys, lower levels in brain, spleen, thymus, and smaller levels in most other tissues. Endogenous furin was detected in lysates from LNCaP and DU145 prostate carcinoma cell lines.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Sucrose
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-Fr: Use at an assay dependent dilution (PMID 20454619).
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Predicted molecular weight: 88 kDa. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
Cellular localization
Golgi apparatus; trans-Golgi network.
Alternative names
- Dibasic processing enzyme antibody
- PACE antibody
- Paired basic amino acid residue cleaving enzyme antibody
see all
Database links
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- Omim: 136950 Human
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
- SwissProt: P23377 Rat
- Unigene: 513153 Human
Anti-Furin antibody - Aminoterminal end images:
Western blot - Anti-Furin antibody - Aminoterminal end (ab28547)
All lanes : Anti-Furin antibody - Aminoterminal end (ab28547) at 1/1000 dilution
Lane 1 : Furin at 0.05 µg
Lane 2 : Furin at 0.01 µg
Lane 3 : Furin at 0.001 µg
Predicted band size : 88 kDa
Observed band size : 88 kDa
Immunohistochemistry (Frozen sections) - Furin antibody - Aminoterminal end (ab28547)
ab28547 staining Furin (red) in mouse endothelial tissue by Immunohistochemistry (Frozen sections).Mice were perfused, tumor and control organs were dissected and frozen in O.C.T. embedding medium. 10 µm cryosections were washed with PBS, fixed for 15 minutes in ice-cold methanol and air-dried. Primary antibody was used at a dilution of 1/1500. The secondary antibody was an Alexa Fluor 594-labeled IgG at a 1/300 dilution. Nuclei are visualized by DAPI staining (blue).
Image from Hajdin K et al, PLoS One. 2010 May 3;5(5):e10445 Fig 4.
Protocols
References for Anti-Furin antibody - Aminoterminal end (ab28547)
This product has been referenced in:
- Hajdin Ket al. Furin targeted drug delivery for treatment of rhabdomyosarcoma in a mouse model. PLoS One 5:e10445 (2010). IHC-Fr; Human.Read more (PubMed: 20454619) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"