Overview

  • Product nameAnti-Furin antibody - Aminoterminal end
    See all Furin primary antibodies
  • Description
    Rabbit polyclonal to Furin - Aminoterminal end
  • Specificityab28547 recognizes subtilisin-like serine protease furin.
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Furin (N terminal).
    (Peptide available as ab41397)

  • Positive control
    • Furin is ubiquitously expressed at low levels, but found in highest concentration in the liver and kidneys, lower levels in brain, spleen, thymus, and smaller levels in most other tissues. Endogenous furin was detected in lysates from LNCaP and DU145 prostate carcinoma cell lines.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody has been peptide-affinity purified.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 88 kDa.

1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection.

IHC-Fr Use at an assay dependent concentration. PubMed: 20454619

Target

  • FunctionFurin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • Tissue specificitySeems to be expressed ubiquitously.
  • Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • DomainContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • Post-translational
    modifications
    The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Anti-Furin antibody - Aminoterminal end images

  • All lanes : Anti-Furin antibody - Aminoterminal end (ab28547) at 1/1000 dilution

    Lane 1 : Furin at 0.05 µg
    Lane 2 : Furin at 0.01 µg
    Lane 3 : Furin at 0.001 µg


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • ab28547 staining Furin (red) in mouse endothelial tissue by Immunohistochemistry (Frozen sections).
    Mice were perfused, tumor and control organs were dissected and frozen in O.C.T. embedding medium. 10 µm cryosections were washed with PBS, fixed for 15 minutes in ice-cold methanol and air-dried. Primary antibody was used at a dilution of 1/1500. The secondary antibody was an Alexa Fluor 594-labeled IgG at a 1/300 dilution. Nuclei are visualized by DAPI staining (blue).

References for Anti-Furin antibody - Aminoterminal end (ab28547)

This product has been referenced in:
  • Sathyamurthy M  et al. Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7. Biotechnol Bioeng N/A:N/A (2014). WB . Read more (PubMed: 25219685) »
  • Cekic M  et al. Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury. Restor Neurol Neurosci 30:115-26 (2012). WB ; Rat . Read more (PubMed: 22232032) »

See all 3 Publications for this product

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