Anti-Furin antibody - C-terminal (ab223510)

Overview

  • Product name
    Anti-Furin antibody - C-terminal
    See all Furin primary antibodies
  • Description
    Goat polyclonal to Furin - C-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Furin aa 777-791 (C terminal) (Cysteine residue). NP_002560.1.
    Sequence:

    C-EDEGRGERTAFIKDQ


    Database link: P09958

  • Positive control
    • WB: HEK-293 overexpressing human Furin with C-terminal Myc tag cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab223510 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).

Target

  • Function
    Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • Tissue specificity
    Seems to be expressed ubiquitously.
  • Sequence similarities
    Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • Domain
    Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • Post-translational
    modifications
    The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Images

  • Lanes 1 - 2 : Anti-Furin antibody - C-terminal (ab223510) at 0.01 µg/ml
    Lane 3 : anti-Myc tag at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) overexpressing human Furin with C-terminal Myc tag, cell lysate in RIPA buffer
    Lane 2 : Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate in RIPA buffer
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) overexpressing human Furin with C-terminal Myc tag, cell lysate in RIPA buffer

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 87 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

References

ab223510 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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