Overview

  • Product name
  • Description
    Mouse monoclonal to FUSIP1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment: MSRYLRPPNT SLFVRNVADD TRSEDLRREF GRYGPIVDVY VPLDFYTRRP RGFAYVQFED VRDAEDALHN LDRKWICGRQ IEIQFAQGDR KTPNQMKAKE, corresponding to amino acids 1 - 100 of human FUSIP1 (AAH05039) with a 26 kDa tag.

  • Positive control
    • Jurkat cell lysate; immunogen

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated. Fully dephosphorylated in mitosis and partially dephosphorylated on heat shock. Isoform 3 is phosphorylated on Ser-168.
  • Cellular localization
    Nucleus speckle. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa SR repressor protein antibody
    • 40 kDa SR-repressor protein antibody
    • Anti TLS associated protein with SR repeats antibody
    • arginine/serine-rich 13A antibody
    • FUS interacting protein serine arginine rich 1 antibody
    • FUS interacting protein serine arginine rich 2 antibody
    • FUS interacting serine arginine rich protein 1 antibody
    • FUS-interacting serine-arginine-rich protein 1 antibody
    • FUSIP1 antibody
    • FUSIP2 antibody
    • NSSR antibody
    • OTTHUMP00000015774 antibody
    • Serine arginine repressor protein 40 kDa antibody
    • Serine/arginine-rich splicing factor 10 antibody
    • SFRS13 antibody
    • SFRS13A antibody
    • Splicing factor antibody
    • Splicing factor arginine serine rich 13 antibody
    • Splicing factor SRp38 antibody
    • SRp38 antibody
    • SRp40 antibody
    • SRrp40 antibody
    • SRS10_HUMAN antibody
    • Srsf10 antibody
    • TASR antibody
    • TASR1 antibody
    • TASR2 antibody
    • TLS associated protein TASR1 antibody
    • TLS associated protein TASR2 antibody
    • TLS associated protein with Ser Arg repeats antibody
    • TLS associated protein with SR repeats antibody
    • TLS associated serine arginine protein 1 antibody
    • TLS associated serine arginine protein 2 antibody
    • TLS associated serine arginine protein antibody
    • TLS associated SR protein antibody
    • TLS-associated protein with Ser-Arg repeats antibody
    • TLS-associated protein with SR repeats antibody
    • TLS-associated serine-arginine protein antibody
    • TLS-associated SR protein antibody
    see all

Anti-FUSIP1 antibody images

  • Anti-FUSIP1 antibody (ab77209) at 1/500 dilution + recombinant tagged FUSIP1 protein (the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 31 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-FUSIP1 antibody (ab77209) at 1/500 dilution + Jurkat cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-FUSIP1 antibody (ab77209)

ab77209 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77209.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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