Overview

  • Product name
  • Description
    Mouse monoclonal to FUSIP1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment: MSRYLRPPNT SLFVRNVADD TRSEDLRREF GRYGPIVDVY VPLDFYTRRP RGFAYVQFED VRDAEDALHN LDRKWICGRQ IEIQFAQGDR KTPNQMKAKE, corresponding to amino acids 1 - 100 of human FUSIP1 (AAH05039) with a 26 kDa tag.

  • Positive control
    • Jurkat cell lysate; immunogen
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab173216 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated. Fully dephosphorylated in mitosis and partially dephosphorylated on heat shock. Isoform 3 is phosphorylated on Ser-168.
  • Cellular localization
    Nucleus speckle. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa SR repressor protein antibody
    • 40 kDa SR-repressor protein antibody
    • Anti TLS associated protein with SR repeats antibody
    • arginine/serine-rich 13A antibody
    • FUS interacting protein serine arginine rich 1 antibody
    • FUS interacting protein serine arginine rich 2 antibody
    • FUS interacting serine arginine rich protein 1 antibody
    • FUS-interacting serine-arginine-rich protein 1 antibody
    • FUSIP1 antibody
    • FUSIP2 antibody
    • NSSR antibody
    • OTTHUMP00000015774 antibody
    • Serine arginine repressor protein 40 kDa antibody
    • Serine/arginine-rich splicing factor 10 antibody
    • SFRS13 antibody
    • SFRS13A antibody
    • Splicing factor antibody
    • Splicing factor arginine serine rich 13 antibody
    • Splicing factor SRp38 antibody
    • SRp38 antibody
    • SRp40 antibody
    • SRrp40 antibody
    • SRS10_HUMAN antibody
    • Srsf10 antibody
    • TASR antibody
    • TASR1 antibody
    • TASR2 antibody
    • TLS associated protein TASR1 antibody
    • TLS associated protein TASR2 antibody
    • TLS associated protein with Ser Arg repeats antibody
    • TLS associated protein with SR repeats antibody
    • TLS associated serine arginine protein 1 antibody
    • TLS associated serine arginine protein 2 antibody
    • TLS associated serine arginine protein antibody
    • TLS associated SR protein antibody
    • TLS-associated protein with Ser-Arg repeats antibody
    • TLS-associated protein with SR repeats antibody
    • TLS-associated serine-arginine protein antibody
    • TLS-associated SR protein antibody
    see all

Images

  • Anti-FUSIP1 antibody (ab77209) at 1/500 dilution + recombinant tagged FUSIP1 protein (the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 31 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-FUSIP1 antibody (ab77209) at 1/500 dilution + Jurkat cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References

ab77209 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Nov 17 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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