Anti-FUSIP1 antibody - C-terminal (ab173216)

Overview

  • Product name
    Anti-FUSIP1 antibody - C-terminal
    See all FUSIP1 primary antibodies
  • Description
    Rabbit polyclonal to FUSIP1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SR Proteins aa 196-225 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O75494

  • Positive control
    • 293 cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab173216 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 31 kDa.

Target

  • Function
    Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated. Fully dephosphorylated in mitosis and partially dephosphorylated on heat shock. Isoform 3 is phosphorylated on Ser-168.
  • Cellular localization
    Nucleus speckle. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa SR repressor protein antibody
    • 40 kDa SR-repressor protein antibody
    • Anti TLS associated protein with SR repeats antibody
    • arginine/serine-rich 13A antibody
    • FUS interacting protein serine arginine rich 1 antibody
    • FUS interacting protein serine arginine rich 2 antibody
    • FUS interacting serine arginine rich protein 1 antibody
    • FUS-interacting serine-arginine-rich protein 1 antibody
    • FUSIP1 antibody
    • FUSIP2 antibody
    • NSSR antibody
    • OTTHUMP00000015774 antibody
    • Serine arginine repressor protein 40 kDa antibody
    • Serine/arginine-rich splicing factor 10 antibody
    • SFRS13 antibody
    • SFRS13A antibody
    • Splicing factor antibody
    • Splicing factor arginine serine rich 13 antibody
    • Splicing factor SRp38 antibody
    • SRp38 antibody
    • SRp40 antibody
    • SRrp40 antibody
    • SRS10_HUMAN antibody
    • Srsf10 antibody
    • TASR antibody
    • TASR1 antibody
    • TASR2 antibody
    • TLS associated protein TASR1 antibody
    • TLS associated protein TASR2 antibody
    • TLS associated protein with Ser Arg repeats antibody
    • TLS associated protein with SR repeats antibody
    • TLS associated serine arginine protein 1 antibody
    • TLS associated serine arginine protein 2 antibody
    • TLS associated serine arginine protein antibody
    • TLS associated SR protein antibody
    • TLS-associated protein with Ser-Arg repeats antibody
    • TLS-associated protein with SR repeats antibody
    • TLS-associated serine-arginine protein antibody
    • TLS-associated SR protein antibody
    see all

Images

  • Anti-FUSIP1 antibody - C-terminal (ab173216) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size: 31 kDa

References

ab173216 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
Yes - 0.3% Triton
Specification
MEF
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 08 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing (10% PAM)
Loading amount
40 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up