Overview

  • Product nameAnti-FUT2 antibody
    See all FUT2 primary antibodies
  • Description
    Mouse monoclonal to FUT2
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: TSKALGPSQL RGMWTINAIG RLGNQMGEYA TLYALAKMNG RPAFIPAQMH STLAPIFRIT LPVLHSATAS RIPWQNYHLN DWMEEEYRHF PGEYVRFTGY , corresponding to amino acids 51-151 of Human FUT2

Properties

Applications

Our Abpromise guarantee covers the use of ab55001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 39 kDa.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCreates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values.
  • Tissue specificitySmall intestine, colon and lung.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyltransferase 11 family.
  • Cellular localizationGolgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2)FT 2 antibody
    • Alpha (1,2) fucosyltransferase antibody
    • Alpha(1 antibody
    • Alpha(1,2)FT 2 antibody
    • Alpha(1,2)FT2 antibody
    • B12QTL1 antibody
    • fucosyltransferase 2 (secretor status included) antibody
    • Fucosyltransferase 2 antibody
    • FUT2 antibody
    • FUT2_HUMAN antibody
    • Galactoside 2-alpha-L-fucosyltransferase 2 antibody
    • GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2 antibody
    • SE antibody
    • SE2 antibody
    • SEC2 antibody
    • Secretor blood group alpha-2-fucosyltransferase antibody
    • Secretor factor antibody
    • Sej antibody
    see all

Anti-FUT2 antibody images



  • Predicted band size : 39 kDa
    FUT2 antibody (ab55001) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • Overlay histogram showing A549 cells stained with ab55001 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55001, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-FUT2 antibody (ab55001)

ab55001 has not yet been referenced specifically in any publications.

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