The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 39 kDa.
Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionCreates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values.
Tissue specificitySmall intestine, colon and lung.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 11 family.
Cellular localizationGolgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
Secretor blood group alpha-2-fucosyltransferase antibody
Secretor factor antibody
Anti-FUT2 antibody images
Western blot - FUT2 antibody (ab55001)
Predicted band size : 39 kDa FUT2 antibody (ab55001) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
Flow Cytometry-Anti-FUT2 antibody(ab55001)
Overlay histogram showing A549 cells stained with ab55001 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55001, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-FUT2 antibody (ab55001)
has not yet been referenced specifically in any publications.
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